# Biohydrogen production from chemical wastewater treatment in biofilm configured reactor operated in periodic discontinuous batch mode by selectively enriched anaerobic mixed consortia.
> 嫌気性混合菌叢を用いたバイオフィルム型逐次回分式反応槽による化学系廃水からの生物学的水素生産


## Abstract

A biofilm-configured periodic discontinuous batch reactor was evaluated for simultaneous molecular hydrogen (H2) production and wastewater remediation using chemical wastewater as the carbon source. Anaerobic mixed consortia were selectively enriched through sequential heat-shock (100°C, 2 h) and acid (pH 3.0, 24 h) pretreatments before reactor inoculation. Operating at mesophilic temperature (28±2°C) under acidophilic conditions, the system achieved a volumetric H2 production rate of 12.89 mmol H2/m³/min with synthetic wastewater and 6.076 mmol H2/m³/min with composite chemical wastewater. COD removal reached 32.4% and 26.7% for synthetic and chemical wastewater, respectively. System stabilization occurred within 37–40 days. Volatile fatty acid concentrations correlated well with H2 evolution (R²=0.961 for synthetic wastewater). The combination of biofilm configuration and batch operation effectively promoted selective enrichment of H2-producing microflora.

### Mechanism

Selective enrichment of H2-producing anaerobes via heat and acid pretreatment, combined with acidophilic operating conditions that suppress methanogenic activity, promotes fermentative H2 generation from organic substrates in wastewater.

## Bibliographic

- **Authors**: Venkata Mohan S, Vijaya Bhaskar Y, Sarma PN
- **Journal**: Water Res
- **Year**: 2007
- **PMID**: [17418367](https://pubmed.ncbi.nlm.nih.gov/17418367/)
- **DOI**: [10.1016/j.watres.2007.02.015](https://doi.org/10.1016/j.watres.2007.02.015)
- **Study type**: other
- **Delivery route**: not specified
- **Effect reported**: not assessed

## Delivery context

The delivery route is not clearly identifiable from this paper. For hydrogen intake, inhalation is the most efficient route; inhalation, however, carries explosion risk (empirical LFL of 10%; high-concentration devices are not recommended).

## Safety notes

The delivery route is not clearly identifiable from this paper. For hydrogen intake, inhalation is the most efficient route; inhalation, however, carries explosion risk (empirical LFL of 10%; high-concentration devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)

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> **Cite as**: H2 Papers — PMID 17418367. https://h2-papers.org/en/papers/17418367
> **Source**: PubMed PMID [17418367](https://pubmed.ncbi.nlm.nih.gov/17418367/)