# Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate.
> 水素がデキストラン硫酸ナトリウム誘発大腸炎の炎症を抑制するメカニズムの検討


## Abstract

A mouse model of inflammatory bowel disease was established by administering 5% dextran sodium sulfate (DSS) in drinking water for 7 days. Animals received DSS alone, DSS combined with hydrogen-rich water, or hydrogen-rich water alone. At day 7, the DSS-only group exhibited significant body weight loss, elevated colitis scores, pathological colon shortening, and increased colonic levels of IL-12, TNF-α, and IL-1β. All of these parameters were markedly reduced in the group receiving hydrogen alongside DSS. Histological examination further confirmed that DSS-associated mucosal tissue destruction and macrophage infiltration were substantially diminished by hydrogen. These findings indicate that hydrogen-rich water can inhibit the progression of DSS-induced colitis in mice, likely through its antioxidant and anti-inflammatory properties.

### Mechanism

Hydrogen's antioxidant activity is proposed to reduce pro-inflammatory cytokine production (IL-12, TNF-α, IL-1β) and macrophage infiltration in colonic tissue, thereby limiting mucosal destruction in DSS-induced colitis.

## Bibliographic

- **Authors**: Kajiya M, Silva MJ, Sato K, Ouhara K, Kawai T
- **Journal**: Biochem Biophys Res Commun
- **Year**: 2009 (2009-08-14)
- **PMID**: [19486890](https://pubmed.ncbi.nlm.nih.gov/19486890/)
- **DOI**: [10.1016/j.bbrc.2009.05.117](https://doi.org/10.1016/j.bbrc.2009.05.117)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 19486890. https://h2-papers.org/en/papers/19486890
> **Source**: PubMed PMID [19486890](https://pubmed.ncbi.nlm.nih.gov/19486890/)