# Hydrogen-rich saline protects myocardium against ischemia/reperfusion injury in rats.
> 水素豊富生理食塩水によるラット心筋虚血再灌流障害に対する保護効果


## Abstract

This rat study examined whether intraperitoneally administered hydrogen-rich saline could reduce cardiac damage following 30 minutes of left anterior descending coronary artery occlusion and 24 hours of reperfusion. Animals receiving hydrogen-rich saline prior to reperfusion showed significantly lower plasma and myocardial malondialdehyde (MDA) levels, reduced 8-hydroxydeoxyguanosine (8-OHdG) in the area at risk, suppressed caspase-3 activity, decreased cardiomyocyte apoptosis, and smaller infarct size compared with controls. Cardiac functional parameters—including left ventricular systolic pressure, diastolic pressure, and the maximum rates of pressure rise and fall (±dP/dt)—were also markedly improved at 24 hours post-reperfusion. These findings indicate that hydrogen-rich saline administration before reperfusion confers measurable cardioprotection in this experimental model.

### Mechanism

Hydrogen-rich saline is proposed to scavenge reactive oxygen species, thereby reducing lipid peroxidation (evidenced by lower MDA and 8-OHdG levels) and suppressing caspase-3-mediated apoptotic signaling, collectively limiting cardiomyocyte death during reperfusion.

## Bibliographic

- **Authors**: Sun Q, Kang Z, Cai J, Liu W, Liu Y, Zhang JH, et al.
- **Journal**: Exp Biol Med (Maywood)
- **Year**: 2009
- **PMID**: [19596825](https://pubmed.ncbi.nlm.nih.gov/19596825/)
- **DOI**: [10.3181/0812-RM-349](https://doi.org/10.3181/0812-RM-349)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 19596825. https://h2-papers.org/en/papers/19596825
> **Source**: PubMed PMID [19596825](https://pubmed.ncbi.nlm.nih.gov/19596825/)