# Experimental verification of protective effect of hydrogen-rich water against cisplatin-induced nephrotoxicity in rats using dynamic contrast-enhanced CT.
> 動的造影CTを用いたシスプラチン誘発腎毒性に対する水素水の腎保護効果の実験的検証


## Abstract

This rat study examined whether hydrogen-rich water could protect against renal dysfunction caused by cisplatin administration, using dynamic contrast-enhanced CT (DCE-CT) as a functional imaging tool. Thirty rats were allocated to three groups: a control group receiving standard water without cisplatin, a non-treatment group receiving cisplatin (3.6 mg/kg intraperitoneally) with standard water, and a treatment group given hydrogen-rich water from 7 days before cisplatin injection. DCE-CT scans were acquired on days 0, 2, 4, and 7 using multidetector CT. Renal contrast clearance per unit volume (K1) and total kidney clearance (K) were derived via the Patlak model. Both K1 and K, normalized to day-0 values, were significantly higher in the hydrogen-rich water group than in the non-treatment group on days 2, 4, and 7, and did not differ significantly from the control group on days 2 and 7. Serum creatinine on day 7 was significantly lower in the hydrogen-rich water group compared with the non-treatment group and was statistically indistinguishable from the control group. These findings indicate that hydrogen-rich water can preserve renal function in cisplatin-exposed rats.

### Mechanism

Hydrogen-rich water is thought to mitigate cisplatin-induced oxidative stress in renal tissue, thereby preserving glomerular filtration capacity and reducing nephrotoxic damage as reflected by improved contrast clearance and lower serum creatinine.

## Bibliographic

- **Authors**: Kitamura A, Kobayashi S, Matsushita T, Fujinawa H, Murase K
- **Journal**: Br J Radiol
- **Year**: 2010
- **PMID**: [20505032](https://pubmed.ncbi.nlm.nih.gov/20505032/)
- **DOI**: [10.1259/bjr/25604811](https://doi.org/10.1259/bjr/25604811)
- **PMC**: [PMC3473599](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3473599/)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 20505032. https://h2-papers.org/en/papers/20505032
> **Source**: PubMed PMID [20505032](https://pubmed.ncbi.nlm.nih.gov/20505032/)