# Saturated hydrogen saline protects the lung against oxygen toxicity.
> 飽和水素生理食塩水による高酸素誘発性肺障害の軽減効果


## Abstract

This animal study examined whether hydrogen-saturated saline could reduce pulmonary damage caused by exposure to greater than 98% oxygen at 2.5 atmospheres absolute (ATA) for five hours in adult male Sprague-Dawley rats. Animals were allocated to three groups: untreated control, normal saline, and hydrogen-saturated saline. Outcome measures included histological assessment via hematoxylin and eosin staining, lung wet-to-dry weight ratio, bronchoalveolar lavage fluid (BALF) protein concentration and total cell count, and lactate dehydrogenase (LDH) activity in both serum and BALF. Compared with the saline group, hydrogen-saturated saline administration resulted in reduced pulmonary edema, lower LDH activity in serum and BALF, and decreased total cell counts and protein levels in BALF. These findings indicate that hydrogen-saturated saline can mitigate hyperoxia-induced lung injury, with oxidative damage suppression identified as a contributing mechanism.

### Mechanism

Molecular hydrogen diffuses rapidly into tissues and cells, scavenging reactive oxygen species and thereby suppressing oxidative damage responsible for hyperoxia-induced alveolar edema, hemorrhage, and cellular injury.

## Bibliographic

- **Authors**: Zheng J, Liu K, Kang Z, Cai J, Liu W, Xu W, et al.
- **Journal**: Undersea Hyperb Med
- **Year**: 2010
- **PMID**: [20568549](https://pubmed.ncbi.nlm.nih.gov/20568549/)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

---

> **Cite as**: H2 Papers — PMID 20568549. https://h2-papers.org/en/papers/20568549
> **Source**: PubMed PMID [20568549](https://pubmed.ncbi.nlm.nih.gov/20568549/)