# Hydrogen-rich saline protects immunocytes from radiation-induced apoptosis.
> 水素富化生理食塩水による放射線誘発免疫細胞アポトーシスへの防護効果


## Abstract

This study examined whether hydrogen-rich saline and medium could protect immune cells from ionizing radiation (IR)-induced damage. In AHH-1 cells, pretreatment with hydrogen-rich medium significantly lowered intracellular hydroxyl radical (•OH) levels as measured by HPF probe. In mouse models, radiation-induced apoptosis in thymocytes and splenocytes was reduced following hydrogen administration, and caspase 3 activation was correspondingly suppressed. Hematological analysis further revealed that radiation-caused reductions in white blood cell and platelet counts were partially restored in hydrogen-treated animals. These findings indicate that molecular hydrogen can mitigate IR-induced immune system damage through free radical scavenging and anti-apoptotic mechanisms.

### Mechanism

Molecular hydrogen scavenges hydroxyl radicals (•OH), thereby suppressing caspase 3 activation and reducing radiation-induced apoptosis in thymocytes, splenocytes, and circulating immune cells.

## Bibliographic

- **Authors**: Yang Y, Li B, Liu C, Chuai Y, Lei J, Gao F, et al.
- **Journal**: Med Sci Monit
- **Year**: 2012
- **PMID**: [22460088](https://pubmed.ncbi.nlm.nih.gov/22460088/)
- **DOI**: [10.12659/msm.882616](https://doi.org/10.12659/msm.882616)
- **PMC**: [PMC3560832](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560832/)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 22460088. https://h2-papers.org/en/papers/22460088
> **Source**: PubMed PMID [22460088](https://pubmed.ncbi.nlm.nih.gov/22460088/)