# Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing oxidative stress.
> 水素富化生理食塩水による外傷性脳損傷ラットモデルでの酸化ストレス軽減と神経保護効果


## Abstract

This study examined the protective capacity of intraperitoneally administered hydrogen-rich saline (HS) in a rat traumatic brain injury (TBI) model induced by controlled cortical impact. Animals received varying doses of HS at 5 minutes post-injury. Compared with untreated TBI rats, HS-treated animals showed dose-dependent reductions in blood-brain barrier permeability, brain edema, lesion volume, and neurological deficits. Analysis of brain tissue revealed elevated endogenous antioxidant enzyme activity and decreased levels of oxidative products following HS administration. These findings indicate that hydrogen-rich saline can mitigate TBI-associated pathology through suppression of oxidative stress, suggesting molecular hydrogen as a candidate intervention for brain injury.

### Mechanism

Hydrogen-rich saline selectively scavenges toxic reactive oxygen species, particularly hydroxyl radicals, while enhancing endogenous antioxidant enzyme activity in brain tissue, thereby reducing oxidative damage following traumatic brain injury.

## Bibliographic

- **Authors**: Ji X, Tian Y, Xie K, Liu W, Qu Y, Fei Z
- **Journal**: J Surg Res
- **Year**: 2012
- **PMID**: [22475349](https://pubmed.ncbi.nlm.nih.gov/22475349/)
- **DOI**: [10.1016/j.jss.2011.12.038](https://doi.org/10.1016/j.jss.2011.12.038)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 22475349. https://h2-papers.org/en/papers/22475349
> **Source**: PubMed PMID [22475349](https://pubmed.ncbi.nlm.nih.gov/22475349/)