# Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid hemorrhage in rats.
> クモ膜下出血後の脳血管攣縮に対する水素富化生理食塩水の保護効果：ラット実験モデルによる検討


## Abstract

Cerebral vasospasm (CV) following subarachnoid hemorrhage (SAH) is a serious complication with incompletely understood mechanisms. This rat study examined whether intraperitoneal hydrogen-rich saline (HS; 5 ml/kg) administered immediately and 24 hours after cisterna magna blood injection could reduce SAH-induced CV. At 48 hours post-SAH, HS-treated animals showed improved neurological scores and reduced morphological vasospasm of the basilar artery as assessed by light and transmission electron microscopy. Biochemical analyses revealed decreased lipid peroxidation, elevated antioxidant enzyme activity, and lower proinflammatory cytokine concentrations in the basilar artery. These findings suggest that molecular hydrogen exerts neuroprotective effects in the context of SAH-induced CV, with the mechanism likely involving suppression of vascular oxidative stress and inflammatory signaling.

### Mechanism

Molecular hydrogen selectively scavenges hydroxyl radicals, thereby reducing lipid peroxidation and proinflammatory cytokine levels while enhancing antioxidant enzyme activity, collectively attenuating basilar artery vasospasm after SAH.

## Bibliographic

- **Authors**: Hong Y, Guo S, Chen SL, Sun C, Zhang JH, Sun X
- **Journal**: J Neurosci Res
- **Year**: 2012
- **PMID**: [22589232](https://pubmed.ncbi.nlm.nih.gov/22589232/)
- **DOI**: [10.1002/jnr.22739](https://doi.org/10.1002/jnr.22739)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 22589232. https://h2-papers.org/en/papers/22589232
> **Source**: PubMed PMID [22589232](https://pubmed.ncbi.nlm.nih.gov/22589232/)