# Treatment of hydrogen molecule abates oxidative stress and alleviates bone loss induced by modeled microgravity in rats.
> 模擬微小重力環境下のラットにおける水素分子投与による酸化ストレス軽減と骨量減少抑制効果


## Abstract

Using a hindlimb suspension (HLS) rat model to simulate microgravity, this study examined the effects of hydrogen-rich water (HW) administered over 6 weeks. HW prevented HLS-induced declines in bone mineral density, ultimate load, stiffness, and energy absorption in both femur and lumbar vertebra. Elevated malondialdehyde and peroxynitrite levels, along with reduced total sulfhydryl content, were also normalized by HW. In MC3T3-E1 osteoblast-like cells exposed to simulated microgravity via a rotary wall vessel bioreactor, hydrogen-rich medium (HRM) suppressed ROS accumulation, restored osteoblastic differentiation, reduced the RANKL/OPG ratio, and attenuated iNOS upregulation and Erk1/2 phosphorylation. In RAW264.7 macrophage-derived cells, HRM inhibited osteoclastic differentiation and osteoclastogenesis under the same conditions. These findings suggest that molecular hydrogen may serve as a nutritional countermeasure against microgravity-associated bone deterioration.

### Mechanism

Molecular hydrogen selectively scavenges ROS and peroxynitrite, restores osteoblast differentiation by normalizing the RANKL/OPG ratio and suppressing Erk1/2 phosphorylation, and inhibits osteoclast differentiation and osteoclastogenesis under microgravity conditions.

## Bibliographic

- **Authors**: Sun YP, Shuang F, Chen DM, Zhou RB
- **Journal**: Osteoporos Int
- **Year**: 2013
- **PMID**: [22648000](https://pubmed.ncbi.nlm.nih.gov/22648000/)
- **DOI**: [10.1007/s00198-012-2028-4](https://doi.org/10.1007/s00198-012-2028-4)
- **Study type**: animal study
- **Delivery route**: mixed routes
- **Effect reported**: positive

## Delivery context

This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 22648000. https://h2-papers.org/en/papers/22648000
> **Source**: PubMed PMID [22648000](https://pubmed.ncbi.nlm.nih.gov/22648000/)