# Therapeutic effects of hydrogen saturated saline on rat diabetic model and insulin resistant model via reduction of oxidative stress.
> 水素飽和生理食塩水がラット糖尿病・インスリン抵抗性モデルに及ぼす酸化ストレス軽減効果


## Abstract

Using rat models of diabetes mellitus (induced by high-fat/high-carbohydrate diet plus low-dose streptozotocin) and insulin resistance (induced by high-glucose/high-fat diet), this study examined the effects of daily hydrogen-saturated saline administration over eight weeks. Compared with both pioglitazone-treated and normal saline-treated control groups, hydrogen-saturated saline produced greater reductions in blood glucose and lipid levels and improved insulin sensitivity. Oxidative stress markers showed a significant decrease in malondialdehyde (MDA) alongside elevated superoxide dismutase (SOD) and glutathione (GSH) levels. These findings suggest that hydrogen-saturated saline may ameliorate insulin resistance and diabetic metabolic disturbances primarily through suppression of oxidative stress and enhancement of endogenous antioxidant defenses.

### Mechanism

Hydrogen-saturated saline is proposed to scavenge reactive oxygen species, thereby reducing lipid peroxidation (MDA) and upregulating antioxidant enzymes SOD and GSH, which collectively attenuate oxidative stress and restore insulin sensitivity.

## Bibliographic

- **Authors**: Wang Q, Zha XJ, Kang Z, Xu MJ, Huang Q, Zou DJ
- **Journal**: Chin Med J (Engl)
- **Year**: 2012
- **PMID**: [22800834](https://pubmed.ncbi.nlm.nih.gov/22800834/)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 22800834. https://h2-papers.org/en/papers/22800834
> **Source**: PubMed PMID [22800834](https://pubmed.ncbi.nlm.nih.gov/22800834/)