# Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells.
> LPS活性化網膜ミクログリア細胞におけるmiR-9・miR-21・miR-199発現に対する水素分子の調節作用


## Abstract

Retinal microglia cells were stimulated with lipopolysaccharide (LPS) and subsequently exposed to hydrogen-saturated medium or control medium. qRT-PCR analysis revealed that hydrogen exposure led to marked decreases in miR-9 and miR-21 levels, while miR-199 expression was elevated. Western blotting of LPS-induced signaling proteins showed reduced Myd88 and IKK-β levels following hydrogen treatment, along with increased PDCD4 expression; NF-κB levels remained unchanged. These findings suggest that molecular hydrogen modulates inflammatory signaling in retinal microglia through coordinated regulation of specific miRNAs, providing a mechanistic basis for its protective effects against inflammatory injury in retinal tissue.

### Mechanism

Molecular hydrogen suppresses miR-9 and miR-21 while upregulating miR-199, resulting in reduced Myd88 and IKK-β expression and increased PDCD4, thereby attenuating upstream NF-κB inflammatory signaling in LPS-activated retinal microglia.

## Bibliographic

- **Authors**: Liu GD, Zhang H, Wang L, Han Q, Zhou SF, Liu P
- **Journal**: Int J Ophthalmol
- **Year**: 2013
- **PMID**: [23826519](https://pubmed.ncbi.nlm.nih.gov/23826519/)
- **DOI**: [10.3980/j.issn.2222-3959.2013.03.05](https://doi.org/10.3980/j.issn.2222-3959.2013.03.05)
- **PMC**: [PMC3693006](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693006/)
- **Study type**: in vitro study
- **Delivery route**: in vitro
- **Effect reported**: positive

## Delivery context

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

## Safety notes

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 23826519. https://h2-papers.org/en/papers/23826519
> **Source**: PubMed PMID [23826519](https://pubmed.ncbi.nlm.nih.gov/23826519/)