# Hydrogen protects auditory hair cells from cisplatin-induced free radicals.
> シスプラチン誘発性フリーラジカルから聴覚有毛細胞を保護する水素の効果


## Abstract

Cisplatin is a commonly used chemotherapeutic agent whose maximum dosage is frequently restricted by its ototoxic side effects, which are thought to involve reactive oxygen species (ROS) generated through cochlea-specific enzyme activation. This in vitro study used mouse cochlear explants cultured in cisplatin-containing medium at varying concentrations, with hydrogen gas dissolved directly into the culture medium. After 48 hours of incubation, phalloidin staining was used to quantify surviving auditory hair cells. Cisplatin produced dose-dependent hair cell loss, while hydrogen gas supplementation significantly increased the number of surviving cells. Hydroxyphenyl fluorescein (HPF) staining of the spiral ganglion further demonstrated that hydroxyl radical formation was markedly reduced in hydrogen-treated cochleae. These findings indicate that molecular hydrogen can mitigate cisplatin-induced oxidative damage to inner ear tissues.

### Mechanism

Molecular hydrogen acts as a selective antioxidant, scavenging hydroxyl radicals generated by cisplatin through cochlea-specific enzyme activation, thereby reducing oxidative damage to auditory hair cells in the inner ear.

## Bibliographic

- **Authors**: Kikkawa YS, Nakagawa T, Taniguchi M, Ito J
- **Journal**: Neurosci Lett
- **Year**: 2014 (2014-09-05)
- **PMID**: [25064701](https://pubmed.ncbi.nlm.nih.gov/25064701/)
- **DOI**: [10.1016/j.neulet.2014.07.025](https://doi.org/10.1016/j.neulet.2014.07.025)
- **Study type**: in vitro study
- **Delivery route**: in vitro
- **Effect reported**: positive

## Delivery context

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

## Safety notes

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 25064701. https://h2-papers.org/en/papers/25064701
> **Source**: PubMed PMID [25064701](https://pubmed.ncbi.nlm.nih.gov/25064701/)