# Protective effect of molecular hydrogen against oxidative stress caused by peroxynitrite derived from nitric oxide in rat retina.
> ラット網膜における一酸化窒素由来ペルオキシナイトライトによる酸化ストレスに対する水素分子の保護効果


## Abstract

Using an organotypic culture system with adult rat retinal tissue, this study examined the effects of H2 on cells exposed to nitric oxide-derived oxidative stress via the donor compound S-nitroso-N-acetylpenicillamine (SNAP). H2 preserved mitochondrial membrane potential and reduced apoptotic cell death in retinal tissue. Tyrosine nitration levels and markers of oxidative damage were both diminished in the H2-treated group. Cell counts in the ganglion cell layer and inner nuclear layer, which declined following SNAP exposure, were maintained when H2 was present. These findings indicate that H2 exerts neuroprotective effects on retinal cells, likely through selective scavenging of peroxynitrite, a highly reactive nitrogen species implicated in glaucomatous neurodegeneration.

### Mechanism

H2 selectively scavenges peroxynitrite, thereby reducing tyrosine nitration, preventing mitochondrial membrane potential loss, and suppressing apoptosis in retinal cells exposed to nitric oxide-derived oxidative stress.

## Bibliographic

- **Authors**: Yokota T, Kamimura N, Igarashi T, Takahashi H, Ohta S, Oharazawa H
- **Journal**: Clin Exp Ophthalmol
- **Year**: 2015
- **PMID**: [25801048](https://pubmed.ncbi.nlm.nih.gov/25801048/)
- **DOI**: [10.1111/ceo.12525](https://doi.org/10.1111/ceo.12525)
- **Study type**: in vitro study
- **Delivery route**: in vitro
- **Effect reported**: positive

## Delivery context

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

## Safety notes

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 25801048. https://h2-papers.org/en/papers/25801048
> **Source**: PubMed PMID [25801048](https://pubmed.ncbi.nlm.nih.gov/25801048/)