# The Evaluation and Quantitation of Dihydrogen Metabolism Using Deuterium Isotope in Rats.
> 重水素同位体を用いたラットにおける水素ガス代謝の定量的評価


## Abstract

This study investigated the quantitative metabolic parameters of molecular hydrogen in vivo using intraperitoneally administered deuterium gas as a tracer, with deuterium enrichment measured in the body water pool. Under physiological conditions in rats, approximately 10% of the administered dose underwent oxidation, providing evidence of antioxidant activity. Neither hypoxic conditions nor endotoxin administration altered deuterium oxidation, whereas pure oxygen inhalation led to a reduction in oxidation. In parallel in vitro experiments using bovine heart submitochondrial particles, hydrogen significantly suppressed superoxide generation at Complex I of the mitochondrial respiratory chain. The authors discuss iron-sulfur clusters as potential mediators of reactive oxygen species production and their interaction with dihydrogen as a plausible mechanistic basis for the observed effects.

### Mechanism

Hydrogen is proposed to interact with iron-sulfur clusters in mitochondrial respiratory chain Complex I, thereby reducing superoxide generation. In vivo, approximately 10% of administered hydrogen undergoes oxidation under physiological conditions, consistent with direct antioxidant activity.

## Bibliographic

- **Authors**: Hyspler R, Ticha A, Schierbeek H, Galkin A, Zadak Z
- **Journal**: PLoS One
- **Year**: 2015
- **PMID**: [26103048](https://pubmed.ncbi.nlm.nih.gov/26103048/)
- **DOI**: [10.1371/journal.pone.0130687](https://doi.org/10.1371/journal.pone.0130687)
- **PMC**: [PMC4477931](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477931/)
- **Study type**: animal study
- **Delivery route**: mixed routes
- **Effect reported**: positive

## Delivery context

This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 26103048. https://h2-papers.org/en/papers/26103048
> **Source**: PubMed PMID [26103048](https://pubmed.ncbi.nlm.nih.gov/26103048/)