# Intravenous infusion of H2-saline suppresses oxidative stress and elevates antioxidant potential in Thoroughbred horses after racing exercise.
> サラブレッド競走馬におけるレース後の酸化ストレス抑制と抗酸化能向上に対する水素生理食塩水静脈投与の効果


## Abstract

Intense exhaustive exercise generates reactive oxygen species that can overwhelm endogenous antioxidant defenses, potentially leading to muscle damage and chronic inflammation. This study examined 13 Thoroughbred horses that received either 2 L of intravenous saline containing 0.6 ppm H2 (H2 group, n = 7) or a placebo (n = 6) prior to a high-intensity simulated race. Oxidative stress markers were significantly lower in the H2 group immediately after the race and at 3 and 24 hours post-race, while antioxidant capacity remained unaffected throughout the observation period. Serum creatine kinase, lactate, and uric acid concentrations rose in both groups regardless of treatment. These findings indicate that intravenous H2-saline selectively suppresses exercise-induced oxidative stress in large animals, and provide data relevant to understanding H2 diffusion dynamics from the bloodstream to target tissues over greater anatomical distances than in smaller species.

### Mechanism

Intravenously delivered H2 diffuses passively from the bloodstream to target tissues, selectively scavenging exercise-induced reactive oxygen species and thereby reducing oxidative stress markers without broadly altering antioxidant enzyme capacity.

## Bibliographic

- **Authors**: Yamazaki M, Kusano K, Ishibashi T, Kiuchi M, Koyama K
- **Journal**: Sci Rep
- **Year**: 2015 (2015-10-23)
- **PMID**: [26493164](https://pubmed.ncbi.nlm.nih.gov/26493164/)
- **DOI**: [10.1038/srep15514](https://doi.org/10.1038/srep15514)
- **PMC**: [PMC4616033](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616033/)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 26493164. https://h2-papers.org/en/papers/26493164
> **Source**: PubMed PMID [26493164](https://pubmed.ncbi.nlm.nih.gov/26493164/)