# Sirtuin Type 1 Mediates the Retinal Protective Effect of Hydrogen-Rich Saline Against Light-Induced Damage in Rats.
> 水素富化生理食塩水による光誘発性網膜障害保護におけるSirtuin 1の媒介機序


## Abstract

In a rat model of intense light-induced retinal injury, intraperitoneal administration of hydrogen-rich saline (HRS) for 5 days elevated Sirt1 (Sirtuin Type 1) expression, partially preserved electroretinogram a- and b-wave amplitudes, and reduced outer nuclear layer cell loss. The Sirt1 activator resveratrol reproduced these protective outcomes, while the Sirt1 inhibitor EX-527 and Sirt1-targeting siRNAs each abolished the benefits of HRS, confirming Sirt1 as a necessary mediator. HRS also upregulated Bcl-2 protein and superoxide dismutase activity, and in a Sirt1-dependent fashion reduced Bax expression, cleaved caspase-3 levels, and the lipid peroxidation marker malondialdehyde. Collectively, these findings indicate that HRS confers retinal protection against phototoxic injury primarily through Sirt1-mediated suppression of apoptosis and oxidative stress.

### Mechanism

HRS upregulates Sirt1, which in turn promotes Bcl-2 expression, suppresses Bax and cleaved caspase-3 to inhibit apoptosis, and enhances SOD activity while reducing malondialdehyde, collectively protecting retinal cells from phototoxic oxidative damage.

## Bibliographic

- **Authors**: Qi LS, Yao L, Liu W, Duan WX, Wang B, Zhang LL, et al.
- **Journal**: Invest Ophthalmol Vis Sci
- **Year**: 2015
- **PMID**: [26720481](https://pubmed.ncbi.nlm.nih.gov/26720481/)
- **DOI**: [10.1167/iovs.15-17034](https://doi.org/10.1167/iovs.15-17034)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

---

> **Cite as**: H2 Papers — PMID 26720481. https://h2-papers.org/en/papers/26720481
> **Source**: PubMed PMID [26720481](https://pubmed.ncbi.nlm.nih.gov/26720481/)