# Hydrogen-rich water improves neurological functional recovery in experimental autoimmune encephalomyelitis mice.
> 水素水が実験的自己免疫性脳脊髄炎マウスの神経機能回復に与える影響


## Abstract

Using the experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis (MS), this study examined the effects of hydrogen-rich water (HRW) administered both prophylactically and after disease onset. Prophylactic HRW at concentrations of 0.36 mM and 0.89 mM delayed EAE onset and lowered peak clinical scores. Post-onset administration of 0.89 mM HRW reduced disease severity, central nervous system (CNS) inflammatory cell infiltration, and demyelination. Mechanistically, HRW suppressed CD4+ T lymphocyte infiltration into the CNS and selectively inhibited Th17 cell differentiation while leaving Th1 cell populations unaffected. The authors note that HRW is non-toxic, low-cost, and capable of crossing the blood-brain barrier, suggesting potential utility in MS management.

### Mechanism

HRW appears to reduce CNS demyelination and inflammation by suppressing CD4+ T lymphocyte infiltration and selectively inhibiting Th17 cell differentiation, without altering Th1 cell populations.

## Bibliographic

- **Authors**: Zhao M, Liu M, Pu YY, Wang D, Xie Y, Xue GC, et al.
- **Journal**: J Neuroimmunol
- **Year**: 2016 (2016-05-15)
- **PMID**: [27138092](https://pubmed.ncbi.nlm.nih.gov/27138092/)
- **DOI**: [10.1016/j.jneuroim.2016.03.006](https://doi.org/10.1016/j.jneuroim.2016.03.006)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 27138092. https://h2-papers.org/en/papers/27138092
> **Source**: PubMed PMID [27138092](https://pubmed.ncbi.nlm.nih.gov/27138092/)