# Protective Effects of Hydrogen against Low-Dose Long-Term Radiation-Induced Damage to the Behavioral Performances, Hematopoietic System, Genital System, and Splenic Lymphocytes in Mice.
> 低線量長期放射線照射によるマウスの行動・造血・生殖・脾臓リンパ球への障害に対する水素の防護効果


## Abstract

This mouse study examined whether molecular hydrogen (H₂) could mitigate multi-system damage arising from low-dose long-term gamma radiation (LDLTR). Animals received whole-body irradiation at doses of 0.1, 0.5, 1.0, or 2.0 Gy, and a comprehensive battery of assessments was conducted after the final exposure: body mass monitoring, forced swim test, open field test, chromosome aberration analysis, peripheral blood cell counts, sperm abnormality evaluation, lymphocyte transformation test, and histopathological examination. Across all dose levels, H₂-treated mice showed statistically significant improvements compared with radiation-only controls in behavioral performance, hematopoietic indices, reproductive parameters, and splenic lymphocyte function, indicating that H₂ exerts broad protective effects against LDLTR-induced injury in multiple biological systems.

### Mechanism

H₂ is proposed to reduce radiation-induced oxidative stress and chromosomal aberrations, thereby attenuating functional impairment across hematopoietic, reproductive, and immune compartments following chronic low-dose gamma irradiation.

## Bibliographic

- **Authors**: Guo J, Zhao D, Lei X, Zhao H, Yang Y, Zhang P, et al.
- **Journal**: Oxid Med Cell Longev
- **Year**: 2016
- **PMID**: [27774116](https://pubmed.ncbi.nlm.nih.gov/27774116/)
- **DOI**: [10.1155/2016/1947819](https://doi.org/10.1155/2016/1947819)
- **PMC**: [PMC5059652](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059652/)
- **Study type**: animal study
- **Delivery route**: animal model
- **Effect reported**: positive

## Delivery context

This study is at the animal-experiment stage. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

This study is at the animal-experiment stage. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 27774116. https://h2-papers.org/en/papers/27774116
> **Source**: PubMed PMID [27774116](https://pubmed.ncbi.nlm.nih.gov/27774116/)