# Preadministration of Hydrogen-Rich Water Protects Against Lipopolysaccharide-Induced Sepsis and Attenuates Liver Injury.
> 水素水の前投与がリポ多糖誘発性敗血症および肝障害に対して示す保護効果


## Abstract

Using a murine endotoxin shock model, this study examined whether prior consumption of hydrogen-dissolved water (HW) could confer protection against lipopolysaccharide (LPS)-induced sepsis. Mice given HW for three days before LPS injection showed prolonged survival compared with controls. Hydrogen concentrations rose rapidly in the liver but not in the kidney following HW intake. At 24 hours post-LPS, HW-pretreated animals exhibited reduced apoptosis and lower oxidative stress markers. Additionally, heme oxygenase-1 expression was upregulated while endothelin-1 expression was diminished in the HW group. These findings suggest that HW activates adaptive antioxidant responses in the liver, thereby mitigating acute organ injury associated with sepsis.

### Mechanism

Hydrogen accumulates preferentially in the liver after HW intake, inducing heme oxygenase-1 expression and suppressing endothelin-1, which together reduce LPS-triggered apoptosis and oxidative stress in hepatic tissue.

## Bibliographic

- **Authors**: Iketani M, Ohshiro J, Urushibara T, Takahashi M, Arai T, Kawaguchi H, et al.
- **Journal**: Shock
- **Year**: 2017
- **PMID**: [27918369](https://pubmed.ncbi.nlm.nih.gov/27918369/)
- **DOI**: [10.1097/SHK.0000000000000810](https://doi.org/10.1097/SHK.0000000000000810)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

---

> **Cite as**: H2 Papers — PMID 27918369. https://h2-papers.org/en/papers/27918369
> **Source**: PubMed PMID [27918369](https://pubmed.ncbi.nlm.nih.gov/27918369/)