# Preventive Effects of Drinking Hydrogen-Rich Water on Gingival Oxidative Stress and Alveolar Bone Resorption in Rats Fed a High-Fat Diet.
> 高脂肪食ラットにおける水素水飲用が歯肉酸化ストレスおよび歯槽骨吸収に及ぼす予防的効果


## Abstract

Obesity promotes oxidative stress in gingival tissue, contributing to alveolar bone loss. This animal study investigated whether hydrogen-rich water could counteract these effects in rats rendered obese by a high-fat diet. Male Fischer 344 rats (n = 18) were allocated to three groups: a standard diet with distilled water (control), a high-fat diet with distilled water, and a high-fat diet with hydrogen-rich water. Oxidative DNA damage was quantified via 8-hydroxydeoxyguanosine (8-OHdG) levels in gingival tissue, and alveolar bone mineral density was assessed by micro-computed tomography. High-fat diet animals exhibited elevated gingival 8-OHdG and reduced alveolar bone density relative to controls. Rats receiving hydrogen-rich water showed attenuated body weight gain, lower gingival 8-OHdG concentrations, and less alveolar bone resorption, suggesting that hydrogen-rich water may limit obesity-associated periodontal deterioration.

### Mechanism

Hydrogen-rich water appears to reduce oxidative DNA damage (8-OHdG) in gingival tissue, thereby limiting the obesity-driven cascade that leads to alveolar bone mineral density loss.

## Bibliographic

- **Authors**: Yoneda T, Tomofuji T, Kunitomo M, Ekuni D, Irie K, Azuma T, et al.
- **Journal**: Nutrients
- **Year**: 2017 (2017-01-13)
- **PMID**: [28098768](https://pubmed.ncbi.nlm.nih.gov/28098768/)
- **DOI**: [10.3390/nu9010064](https://doi.org/10.3390/nu9010064)
- **PMC**: [PMC5295108](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295108/)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

---

> **Cite as**: H2 Papers — PMID 28098768. https://h2-papers.org/en/papers/28098768
> **Source**: PubMed PMID [28098768](https://pubmed.ncbi.nlm.nih.gov/28098768/)