# Hydrogen-rich water alleviates the toxicities of different stresses to mycelial growth in Hypsizygus marmoreus.
> 水素水がHypsizygus marmoreus菌糸体の各種ストレス毒性を軽減する効果とその分子機序


## Abstract

This study examined the physiological and molecular effects of hydrogen-rich water (HRW, 0.8 mM H2) on the basidiomycete Hypsizygus marmoreus under three distinct stress conditions: cadmium chloride, sodium chloride, and hydrogen peroxide. HRW application significantly improved mycelial growth and biomass under all three stressors. Malondialdehyde accumulation was markedly reduced, indicating diminished lipid peroxidation. Activities of superoxide dismutase, catalase, and glutathione reductase were elevated alongside their corresponding mRNA levels. In vivo detection confirmed suppression of reactive oxygen species including H2O2 and superoxide anion. Additionally, pyruvate kinase activity and gene expression were upregulated, suggesting enhanced glucose metabolism. The findings indicate that HRW confers broad stress tolerance in this edible fungus through coordinated antioxidant enzyme induction and metabolic activation, paralleling effects previously documented in plant systems.

### Mechanism

HRW elevates the activity and mRNA expression of antioxidant enzymes (SOD, CAT, GR), suppressing ROS accumulation and lipid peroxidation (MDA reduction). Concurrent activation of pyruvate kinase and its gene expression suggests enhanced glucose metabolism, collectively improving stress tolerance in fungal mycelia.

## Bibliographic

- **Authors**: Zhang JH, Hao H, Chen M, Wang H, Feng Z, Chen H
- **Journal**: AMB Express
- **Year**: 2017
- **PMID**: [28565883](https://pubmed.ncbi.nlm.nih.gov/28565883/)
- **DOI**: [10.1186/s13568-017-0406-1](https://doi.org/10.1186/s13568-017-0406-1)
- **PMC**: [PMC5449350](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5449350/)
- **Study type**: in vitro study
- **Delivery route**: in vitro
- **Effect reported**: positive

## Delivery context

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

## Safety notes

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 28565883. https://h2-papers.org/en/papers/28565883
> **Source**: PubMed PMID [28565883](https://pubmed.ncbi.nlm.nih.gov/28565883/)