# [Effect of hydrogen-rich water on the CD34 expression in lesion boundary brain tissue of rats with traumatic brain injury].
> 外傷性脳損傷ラットの病変境界部におけるCD34発現と血管新生に対する水素富化水の影響


## Abstract

Using a rat model of traumatic brain injury (TBI) induced by a modified free-fall impact method, this study examined how intraperitoneally administered hydrogen-rich water (5 mL/kg daily) influenced CD34 expression and capillary formation in perilesional brain tissue. Fifty-four male Sprague-Dawley rats were randomly assigned to sham, TBI, or TBI plus hydrogen-rich water groups, with assessments at 1, 3, and 7 days post-injury. Neurological severity scores were significantly reduced in the hydrogen-rich water group at days 3 and 7 compared with TBI controls. Immunohistochemical analysis revealed a significantly greater number of newly formed capillaries at days 3 and 7 in the hydrogen-rich water group. Western blot analysis showed a marked elevation of CD34 protein at day 7 in the hydrogen-rich water group relative to TBI controls (gray value: 1.36±0.36 vs. 0.74±0.08, P<0.05). Histological examination indicated reduced edema and necrosis in hydrogen-rich water-treated animals. These findings suggest that hydrogen-rich water facilitates CD34-positive cell recruitment to injured tissue, promotes angiogenesis, and supports neurological functional recovery after TBI.

### Mechanism

Hydrogen-rich water appears to enhance the recruitment of CD34-positive progenitor cells to perilesional brain tissue, thereby augmenting neovascularization and supporting neurological recovery following traumatic brain injury.

## Bibliographic

- **Authors**: Liu Y, Lan Q, Wang D, Chen X, Yuan J, Zhang H
- **Journal**: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
- **Year**: 2017
- **PMID**: [28627348](https://pubmed.ncbi.nlm.nih.gov/28627348/)
- **DOI**: [10.3760/cma.j.issn.2095-4352.2017.03.013](https://doi.org/10.3760/cma.j.issn.2095-4352.2017.03.013)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 28627348. https://h2-papers.org/en/papers/28627348
> **Source**: PubMed PMID [28627348](https://pubmed.ncbi.nlm.nih.gov/28627348/)