# Molecular hydrogen modulates gene expression via histone modification and induces the mitochondrial unfolded protein response.
> 水素ガスはヒストン修飾を介した遺伝子発現調節とミトコンドリア・アンフォールドタンパク質応答の誘導をもたらす


## Abstract

Using DNA microarray and gene set enrichment analysis of RNA from liver and lung tissues of rats and mice under dietary stress conditions, this study identified gene sets commonly responsive to molecular hydrogen (H2). H2 was found to activate genes regulated by histone H3K27 methylation status, and RT-qPCR confirmed upregulation of the H3K27-regulated gene Kcnc3 across liver, lung, kidney, and brain. Immunohistochemical and immunoblot analyses revealed alterations in H3K27 methylation in the livers of H2-administered animals. Notably, H2 simultaneously induced expression of the H3K27 demethylase Jmjd3 and genes associated with the mitochondrial unfolded protein response (mtUPR). These findings suggest that H2 influences gene expression through epigenetic histone modification, potentially linking mitochondrial function changes to mtUPR activation via chromatin restructuring.

### Mechanism

H2 induces the H3K27 demethylase Jmjd3, altering histone H3K27 methylation status and thereby activating mitochondrial unfolded protein response (mtUPR)-related gene expression through an epigenetic mechanism.

## Bibliographic

- **Authors**: Sobue S, Inoue C, Hori F, Qiao S, Murate T, Ichihara M
- **Journal**: Biochem Biophys Res Commun
- **Year**: 2017 (2017-11-04)
- **PMID**: [28890349](https://pubmed.ncbi.nlm.nih.gov/28890349/)
- **DOI**: [10.1016/j.bbrc.2017.09.024](https://doi.org/10.1016/j.bbrc.2017.09.024)
- **Study type**: animal study
- **Delivery route**: animal model
- **Effect reported**: positive

## Delivery context

This study is at the animal-experiment stage. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

This study is at the animal-experiment stage. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 28890349. https://h2-papers.org/en/papers/28890349
> **Source**: PubMed PMID [28890349](https://pubmed.ncbi.nlm.nih.gov/28890349/)