# Prevention of ischemia-induced hearing loss by intravenous administration of hydrogen-rich saline in gerbil.
> 水素豊富生理食塩水の静脈内投与による虚血性難聴の予防効果：スナネズミを用いた検討


## Abstract

A transient cochlear ischemia model was established in Mongolian gerbils by bilateral vertebral artery occlusion for 15 minutes. Hydrogen-rich saline (5 mL) was administered intravenously immediately after the ischemic insult, with plain saline serving as the control. Seven days post-ischemia, auditory brainstem response (ABR) thresholds at 8 kHz increased by 24.2±3.8 dB and inner hair cell (IHC) counts at the basal cochlear turn declined by 14.1±1.8% in controls. Animals receiving hydrogen-rich saline showed significantly attenuated cochlear injury: ABR threshold elevation was reduced to 11.7±2.6 dB and IHC loss to 7.5±2.1%. Protective effects were more pronounced at higher frequencies (16 and 32 kHz). No changes in ABR thresholds or cochlear histology were observed in non-ischemic animals given either solution.

### Mechanism

Hydrogen is proposed to scavenge reactive oxygen species generated during cochlear ischemia-reperfusion, thereby limiting oxidative damage to inner hair cells and preserving auditory function.

## Bibliographic

- **Authors**: Ogawa H, Okada M, Shudou M, Gyo K, Hato N
- **Journal**: Neurosci Lett
- **Year**: 2018 (2018-02-05)
- **PMID**: [29223865](https://pubmed.ncbi.nlm.nih.gov/29223865/)
- **DOI**: [10.1016/j.neulet.2017.12.013](https://doi.org/10.1016/j.neulet.2017.12.013)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 29223865. https://h2-papers.org/en/papers/29223865
> **Source**: PubMed PMID [29223865](https://pubmed.ncbi.nlm.nih.gov/29223865/)