# Attenuation of Cardiac Ischaemia-reperfusion Injury by Treatment with Hydrogen-rich Water.
> 水素水投与による心筋虚血再灌流障害の軽減とJAK2-STAT3シグナル経路の関与


## Abstract

Using 20 male Wistar rats, this study investigated how hydrogen-rich water influences myocardial ischaemia-reperfusion injury. Protein chip analysis of left ventricular tissue revealed that 25 proteins spanning five transduction pathways were downregulated in the hydrogen-rich water group. Western blot and immunohistochemical assessments showed elevated p-JAK2/JAK2 and p-STAT3/STAT3 ratios alongside reduced p-STAT1/STAT1 expression in hydrogen-rich water-treated animals compared with controls. TUNEL staining demonstrated a significant reduction in cardiomyocyte apoptosis in the hydrogen-rich water group. These findings indicate that hydrogen-rich water may confer cardioprotection following ischaemia-reperfusion by activating the JAK2-STAT3 signalling axis while suppressing STAT1 phosphorylation.

### Mechanism

Hydrogen-rich water upregulates JAK2 and STAT3 phosphorylation while downregulating STAT1 phosphorylation, thereby reducing cardiomyocyte apoptosis and attenuating myocardial ischaemia-reperfusion injury.

## Bibliographic

- **Authors**: Li XM, Li L, Liu X, Wu J, Sun X, Li Z, et al.
- **Journal**: Curr Mol Med
- **Year**: 2019
- **PMID**: [30907314](https://pubmed.ncbi.nlm.nih.gov/30907314/)
- **DOI**: [10.2174/1566524019666190321113544](https://doi.org/10.2174/1566524019666190321113544)
- **PMC**: [PMC7061975](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061975/)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 30907314. https://h2-papers.org/en/papers/30907314
> **Source**: PubMed PMID [30907314](https://pubmed.ncbi.nlm.nih.gov/30907314/)