# Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley.
> 水素水処理が発芽黒大麦の栄養成分および抗酸化特性に与える影響


## Abstract

This study examined the effects of hydrogen-rich water (HRW, 2 ppm) on sprouted black barley (Hordeum distichum L.) during germination processing. HRW treatment significantly elevated both germination rate and growth rate compared with ultra-pure water controls (P < 0.05). Chemical composition analysis revealed that HRW altered phytochemical distribution, including changes in guanosine ionic strength, and increased concentrations of free vanillic acid, coumaric acid, sinapic acid, conjugated sinapic acid, calcium, and iron. Hydroxyl radical scavenging capacity was also enhanced. Conversely, contents of protein, fat, starch, and dietary fiber were reduced relative to the ultra-pure water group. These findings suggest that HRW application during grain sprouting can improve germination efficiency and elevate bioactive phytochemical levels in the resulting product.

### Mechanism

HRW is proposed to modify phytochemical distribution during germination, elevating phenolic acid concentrations (vanillic, coumaric, and sinapic acids) and mineral levels (Ca, Fe), thereby enhancing hydroxyl radical scavenging capacity in sprouted black barley.

## Bibliographic

- **Authors**: Guan Q, Ding XW, Jiang R, Ouyang PL, Gui J, Feng L, et al.
- **Journal**: Food Chem
- **Year**: 2019 (2019-11-30)
- **PMID**: [31279124](https://pubmed.ncbi.nlm.nih.gov/31279124/)
- **DOI**: [10.1016/j.foodchem.2019.125095](https://doi.org/10.1016/j.foodchem.2019.125095)
- **Study type**: in vitro study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 31279124. https://h2-papers.org/en/papers/31279124
> **Source**: PubMed PMID [31279124](https://pubmed.ncbi.nlm.nih.gov/31279124/)