# Molecular hydrogen suppresses free-radical-induced cell death by mitigating fatty acid peroxidation and mitochondrial dysfunction.
> 分子状水素による脂肪酸過酸化およびミトコンドリア機能障害の抑制を介したフリーラジカル誘発細胞死の防御


## Abstract

Using the human acute monocytic leukemia cell line THP-1, this in vitro study examined how molecular hydrogen (H2) influences cytotoxicity induced by tert-butyl hydroperoxide, a free-radical generator. Cell membrane integrity was assessed via lactate dehydrogenase release and dual fluorescent staining. Fatty acid peroxidation was quantified with Liperfluo and C11-BODIPY probes, while mitochondrial oxidoreductase activity was evaluated by the alamarBlue resazurin-reduction assay and membrane potential by tetramethylrhodamine methyl ester. H2 exposure reduced fatty acid peroxidation, limited increases in membrane permeability, preserved mitochondrial oxidoreductase activity and membrane potential, and decreased propidium-iodide-positive (dead) cell counts. The findings indicate that H2 confers cytoprotection against free-radical-induced death primarily through attenuation of lipid peroxidation and maintenance of mitochondrial integrity.

### Mechanism

H2 reduces free-radical-driven fatty acid peroxidation and preserves mitochondrial oxidoreductase activity and membrane potential, thereby preventing cell death in oxidatively stressed cells.

## Bibliographic

- **Authors**: Iuchi K, Nishimaki K, Kamimura N, Ohta S
- **Journal**: Can J Physiol Pharmacol
- **Year**: 2019
- **PMID**: [31295412](https://pubmed.ncbi.nlm.nih.gov/31295412/)
- **DOI**: [10.1139/cjpp-2018-0741](https://doi.org/10.1139/cjpp-2018-0741)
- **Study type**: in vitro study
- **Delivery route**: in vitro
- **Effect reported**: positive

## Delivery context

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

## Safety notes

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 31295412. https://h2-papers.org/en/papers/31295412
> **Source**: PubMed PMID [31295412](https://pubmed.ncbi.nlm.nih.gov/31295412/)