# [Protective effect of hydrogen-rich water on oxidative stress cell model and the impact of the phosphatidylinositol 3 kinase/protein kinase B pathway].
> 水素水によるマウスアストロサイトの酸化ストレス障害に対する保護効果とPI3K/Aktシグナル経路への影響


## Abstract

This in vitro study examined how hydrogen-rich water (HRW) influences oxidative stress injury in mouse astrocytes and whether the PI3K/Akt signaling pathway is involved. An astrocyte injury model was established using 2.50 µmol/L hydrogen peroxide (H2O2) for 20 minutes, which reduced cell viability to approximately 50%. Pretreatment with 50 µmol/L HRW for 9 hours yielded the highest cell survival rate. In experiment two, H2O2 exposure significantly reduced PI3K and Akt mRNA and protein expression, including phosphorylated Akt (p-Akt). HRW pretreatment reversed these reductions, significantly elevating PI3K mRNA, Akt mRNA, and PI3K, Akt, p-Akt protein levels compared with the H2O2 injury group (all P < 0.05). Co-treatment with the PI3K/Akt inhibitor wortmannin (200 nmol/L) abolished the protective effects of HRW, returning expression levels to those seen in the injury group. These findings indicate that HRW activates the PI3K/Akt pathway to confer antioxidant protection in astrocytes.

### Mechanism

Hydrogen-rich water activates the PI3K/Akt signaling pathway, upregulating PI3K, Akt, and phosphorylated Akt expression, thereby enhancing the antioxidant capacity of astrocytes under oxidative stress conditions.

## Bibliographic

- **Authors**: Lu R, Liu Y, Wang D
- **Journal**: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
- **Year**: 2019
- **PMID**: [31315738](https://pubmed.ncbi.nlm.nih.gov/31315738/)
- **DOI**: [10.3760/cma.j.issn.2095-4352.2019.06.020](https://doi.org/10.3760/cma.j.issn.2095-4352.2019.06.020)
- **Study type**: in vitro study
- **Delivery route**: in vitro
- **Effect reported**: positive

## Delivery context

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

## Safety notes

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 31315738. https://h2-papers.org/en/papers/31315738
> **Source**: PubMed PMID [31315738](https://pubmed.ncbi.nlm.nih.gov/31315738/)