# Molecular hydrogen regulates PTEN-AKT-mTOR signaling via ROS to alleviate peritoneal dialysis-related peritoneal fibrosis.
> 分子状水素はROSを介したPTEN-AKT-mTORシグナル経路の調節により腹膜透析関連腹膜線維化を軽減する


## Abstract

Peritoneal dialysis is used by approximately 11% of end-stage renal disease patients globally, yet prolonged use leads to peritoneal fibrosis. This study established a high-glucose-induced peritoneal fibrosis mouse model via intraperitoneal injection of high-glucose dialysate, followed by intervention with hydrogen-rich dialysate. Parallel in vitro experiments were conducted using MeT-5A mesothelial cells. Both in vivo and in vitro findings demonstrated that molecular hydrogen effectively suppressed the progression of high-glucose-induced peritoneal fibrosis. Mechanistically, hydrogen eliminated intracellular reactive oxygen species and blocked activation of the PTEN/AKT/mTOR signaling pathway. These results indicate that the ROS/PTEN/AKT/mTOR axis mediates the anti-fibrotic action of molecular hydrogen in the peritoneal environment.

### Mechanism

Molecular hydrogen selectively scavenges intracellular ROS, thereby preventing activation of the PTEN/AKT/mTOR signaling cascade and inhibiting high-glucose-driven peritoneal fibrosis progression.

## Bibliographic

- **Authors**: Lu H, Chen W, Liu W, Si Y, Zhao T, Lai X, et al.
- **Journal**: FASEB J
- **Year**: 2020
- **PMID**: [31930571](https://pubmed.ncbi.nlm.nih.gov/31930571/)
- **DOI**: [10.1096/fj.201901981R](https://doi.org/10.1096/fj.201901981R)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 31930571. https://h2-papers.org/en/papers/31930571
> **Source**: PubMed PMID [31930571](https://pubmed.ncbi.nlm.nih.gov/31930571/)