# Ultrathin cellulose film coating of porous alumina membranes for adsorption of superoxide dismutase.
> 多孔質アルミナ膜への超薄セルロース膜コーティングによるスーパーオキシドジスムターゼ吸着


## Abstract

This study describes a method for depositing ultrathin cellulose films onto the three-dimensional pore surfaces of anodic aluminum oxide membranes. Pore walls were first pre-coated with a polyelectrolyte multilayer (approximately 5.0 nm thick), followed by cellulose deposition from N-methylmorpholine oxide solution, yielding a film of approximately 15.0 nm composed of dense cellulose nanoparticles averaging 5.5 ± 1.4 nm in diameter. The porous architecture of the membrane was maintained throughout the process. Subsequent dissolution of the alumina template under acidic conditions produced flexible nanotubes with an outer diameter of approximately 200 nm. The cellulose-coated surfaces provided abundant hydrogen bonding sites and achieved a superoxide dismutase adsorption capacity of approximately 1.45 mg/m². This approach extends cellulose film coating capability from flat two-dimensional substrates to complex three-dimensional structures, offering improved protein adsorption performance.

### Mechanism

The dense hydrogen bonding sites on the cellulose film surface facilitate nonspecific adsorption of superoxide dismutase protein molecules, enabling a high adsorption capacity of approximately 1.45 mg/m² on the three-dimensional porous substrate.

## Bibliographic

- **Authors**: Gu Y, Huang J
- **Journal**: J Mater Chem B
- **Year**: 2013 (2013-11-07)
- **PMID**: [32261187](https://pubmed.ncbi.nlm.nih.gov/32261187/)
- **DOI**: [10.1039/c3tb20725k](https://doi.org/10.1039/c3tb20725k)
- **Study type**: in vitro study
- **Delivery route**: in vitro
- **Effect reported**: not assessed

## Delivery context

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

## Safety notes

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 32261187. https://h2-papers.org/en/papers/32261187
> **Source**: PubMed PMID [32261187](https://pubmed.ncbi.nlm.nih.gov/32261187/)