# A study on the protective effect of molecular hydrogen on osteoradionecrosis of the jaw in rats.
> ラットの顎骨放射線壊死に対する分子状水素の保護効果に関する研究


## Abstract

This animal study examined whether hydrogen pretreatment could protect against radiation-induced osteoradionecrosis of the jaw (ORNJ) using Sprague-Dawley rats and bone marrow-derived mesenchymal stem cells (BMSCs). BMSCs received 4 Gy irradiation after hydrogen pretreatment; rats received 7 Gy per fraction over five daily fractions. In irradiated BMSCs, hydrogen pretreatment significantly reduced reactive oxygen species (ROS) production, improved cell viability (P=0.025 vs. irradiation alone, P=0.001), and enhanced differentiation potential. In vivo, hydrogen-rich saline-treated rats showed notable improvements in occlusion, salivation, alopecia, and oral ulceration, along with reduced bone necrosis on micro-CT and histology. Myofibroblast accumulation in fibrotic medulla and around sequestra was also diminished in the hydrogen-pretreated group.

### Mechanism

Hydrogen scavenges radiation-induced reactive oxygen species in BMSCs, preserving cell viability and differentiation capacity, while reducing myofibroblast accumulation in irradiated bone marrow, thereby limiting the progression of jaw osteoradionecrosis.

## Bibliographic

- **Authors**: Chen Y, Zong C, Jia J, Liu Y, Zhang Z, Cai B, et al.
- **Journal**: Int J Oral Maxillofac Surg
- **Year**: 2020
- **PMID**: [32451233](https://pubmed.ncbi.nlm.nih.gov/32451233/)
- **DOI**: [10.1016/j.ijom.2020.04.011](https://doi.org/10.1016/j.ijom.2020.04.011)
- **Study type**: animal study
- **Delivery route**: mixed routes
- **Effect reported**: positive

## Delivery context

This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 32451233. https://h2-papers.org/en/papers/32451233
> **Source**: PubMed PMID [32451233](https://pubmed.ncbi.nlm.nih.gov/32451233/)