# Metabolomics Analysis of the Effect of Hydrogen-Rich Water on Myocardial Ischemia-Reperfusion Injury in Rats.
> 水素水がラット心筋虚血再灌流傷害に及ぼす影響のメタボロミクス解析


## Abstract

Using a Langendorff-perfused isolated rat heart model, this study examined how hydrogen-rich water (HRW) influences myocardial tissue metabolism during ischemia-reperfusion injury (MIRI). Twelve rats were divided equally into HRW and control groups; hearts were perfused with Krebs-Ringer solution with or without HRW at 37°C. LC-MS-based metabolomics combined with PCA, PLS-DA, and OPLS-DA statistical approaches identified metabolites differing significantly between groups (VIP ≥ 1, p < 0.05). Seven MIRI-associated signaling pathways were uncovered, including glycerophospholipid metabolism, glycosylphosphatidylinositol-anchored biosynthesis, and purine metabolism. Ten candidate biomarkers were identified, among them phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine. The findings suggest that HRW modulates metabolic dysregulation in ischemia-reperfusion-injured myocardium through multiple interconnected pathways, resulting in reduced cardiac injury in the isolated heart preparation.

### Mechanism

HRW modulates at least seven MIRI-related metabolic pathways—including glycerophospholipid metabolism, GPI-anchored biosynthesis, and purine metabolism—normalizing phospholipid biomarkers such as phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine to restore myocardial metabolic homeostasis.

## Bibliographic

- **Authors**: Li L, Liu TT, Liu L, Li SC, Zhang Z, Zhou YN, et al.
- **Journal**: J Bioenerg Biomembr
- **Year**: 2020
- **PMID**: [32472432](https://pubmed.ncbi.nlm.nih.gov/32472432/)
- **DOI**: [10.1007/s10863-020-09835-7](https://doi.org/10.1007/s10863-020-09835-7)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 32472432. https://h2-papers.org/en/papers/32472432
> **Source**: PubMed PMID [32472432](https://pubmed.ncbi.nlm.nih.gov/32472432/)