# Hydrogen-rich water promotes elongation of hypocotyls and roots in plants through mediating the level of endogenous gibberellin and auxin.
> 水素水が植物の胚軸・根の伸長に与える影響：内生ジベレリンおよびオーキシン濃度の変動を介したメカニズムの検討


## Abstract

This study examined the effects of hydrogen-rich water (HRW) on vegetable seedling growth. Treatment with 480 μM H2 (60% of saturated concentration) significantly increased fresh weight, hypocotyl length, and root length in mung bean seedlings compared with controls. In dark-grown seedlings, HRW elevated endogenous IAA and GA3 levels. Exogenous GA3 enhanced hypocotyl elongation specifically, whereas uniconazole, a GA3 biosynthesis inhibitor, suppressed HRW-induced hypocotyl elongation without affecting roots. IAA application amplified HRW-induced elongation in both organs, while the IAA biosynthesis inhibitor TIBA abolished these effects. Growth-promoting outcomes were also observed in cucumber and radish seedlings, indicating a general mechanism applicable across species.

### Mechanism

HRW elevates GA3 content in hypocotyls and IAA content in roots, stimulating cell elongation in each organ through distinct phytohormone pathways and thereby promoting overall seedling growth.

## Bibliographic

- **Authors**: Wu QF, Su N, Huang X, Ling X, Yu M, Cui J, et al.
- **Journal**: Funct Plant Biol
- **Year**: 2020
- **PMID**: [32522330](https://pubmed.ncbi.nlm.nih.gov/32522330/)
- **DOI**: [10.1071/FP19107](https://doi.org/10.1071/FP19107)
- **Study type**: other
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

---

> **Cite as**: H2 Papers — PMID 32522330. https://h2-papers.org/en/papers/32522330
> **Source**: PubMed PMID [32522330](https://pubmed.ncbi.nlm.nih.gov/32522330/)