# Protective Effects of a Hydrogen-Rich Preservation Solution in a Canine Lung Transplantation Model.
> イヌ肺移植モデルにおける水素富化保存液の虚血再灌流傷害に対する保護効果


## Abstract

Using a canine orthotopic left lung transplantation model (n=10 beagles), researchers compared hydrogen-rich ET-Kyoto solution (H2 group, n=5) with standard ET-Kyoto solution (control group, n=5) for donor lung flushing and cold preservation over 23 hours at 4°C, followed by 4 hours of reperfusion. Gas exchange assessment after right pulmonary artery clamping revealed significantly higher arterial oxygen partial pressure (P=0.045) and lower carbon dioxide partial pressure (P<0.001) in the H2 group. The wet-to-dry weight ratio, an indicator of pulmonary edema, was significantly reduced in the H2 group (P=0.032). Histological analysis further confirmed markedly fewer signs of lung injury and a lower count of apoptotic cells in the H2 group (both P<0.001). These findings indicate that hydrogen-enriched preservation solution can mitigate ischemia-reperfusion injury in transplanted lungs.

### Mechanism

Hydrogen dissolved in the preservation solution is thought to scavenge reactive oxygen species generated during reperfusion, thereby reducing oxidative stress-driven apoptosis and attenuating subsequent lung tissue injury after transplantation.

## Bibliographic

- **Authors**: Kayawake H, Chen-Yoshikawa TF, Saito M, Yamagishi H, Yoshizawa A, Hirano S, et al.
- **Journal**: Ann Thorac Surg
- **Year**: 2021
- **PMID**: [32649946](https://pubmed.ncbi.nlm.nih.gov/32649946/)
- **DOI**: [10.1016/j.athoracsur.2020.05.076](https://doi.org/10.1016/j.athoracsur.2020.05.076)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 32649946. https://h2-papers.org/en/papers/32649946
> **Source**: PubMed PMID [32649946](https://pubmed.ncbi.nlm.nih.gov/32649946/)