# Drinking Molecular Hydrogen Water Is Beneficial to Cardiovascular Function in Diet-Induced Obesity Mice.
> 高脂肪食誘発肥満マウスにおける水素水摂取の心血管機能への有益な効果


## Abstract

This study examined the effects of molecular hydrogen water (MHW) consumption on cardiovascular parameters in high-fat diet-induced obese (DIO) mice. After 2 weeks of MHW intake, blood glucose and body weight remained unchanged; however, heart weight was reduced. Cardiac hypertrophy was ameliorated, cardiomyocyte width decreased, capillaries and arterioles dilated, myocardial eNOS-Ser-1177 phosphorylation was activated, and left ventricular function was restored. In white and brown adipose tissues, a histological shift from hypertrophy to hyperplasia was observed, accompanied by upregulation of thermogenic and cardiovascular-protective genes in brown adipose tissue. Colony formation assays of bone-marrow-derived endothelial progenitor cells (EPCs) demonstrated enhanced expansion, differentiation, and mobilization, suggesting a role in vascular homeostasis maintenance. Collectively, these findings indicate that MHW intake confers cardiovascular protection in a metabolic syndrome model.

### Mechanism

MHW activates eNOS-Ser-1177 phosphorylation in myocardial tissue and promotes the expansion, differentiation, and mobilization of bone-marrow-derived endothelial progenitor cells, thereby supporting vascular homeostasis and reducing cardiac hypertrophy in obese mice.

## Bibliographic

- **Authors**: Masuda H, Sato A, Miyata K, Shizuno T, Oyamada A, Ishiwata K, et al.
- **Journal**: Biology (Basel)
- **Year**: 2021 (2021-04-23)
- **PMID**: [33922704](https://pubmed.ncbi.nlm.nih.gov/33922704/)
- **DOI**: [10.3390/biology10050364](https://doi.org/10.3390/biology10050364)
- **PMC**: [PMC8146054](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146054/)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 33922704. https://h2-papers.org/en/papers/33922704
> **Source**: PubMed PMID [33922704](https://pubmed.ncbi.nlm.nih.gov/33922704/)