# Effects of hydrogen-rich water and ascorbic acid treatment on spontaneously hypertensive rats.
> 水素水およびアスコルビン酸併用が自然発症高血圧ラットに及ぼす影響


## Abstract

This study investigated the effects of hydrogen-rich water (HW) and HW supplemented with 0.1% ascorbic acid (HWA) in spontaneously hypertensive rats (SHR). Under a normal diet, HW alone produced no significant change in systolic or diastolic blood pressure, whereas HWA suppressed blood pressure elevation. When SHR were placed on a 4% NaCl-enriched diet to exacerbate hypertension, HWA administration led to a significant reduction in blood pressure compared with distilled water controls. The HWA group also showed a tendency toward lower plasma angiotensin II concentrations, along with significantly reduced urinary volume and urinary sodium excretion. Urinary isoprostane, a marker of oxidative stress, was significantly lower in the HWA group, indicating that attenuation of oxidative stress may underlie the blood pressure-lowering effect. These findings point to a synergistic interaction between HW and ascorbic acid in modulating hypertension-related parameters.

### Mechanism

The combination of hydrogen-rich water and ascorbic acid appears to synergistically reduce oxidative stress, evidenced by decreased urinary isoprostane, and may suppress blood pressure elevation partly through reduced angiotensin II levels.

## Bibliographic

- **Authors**: Kawakami K, Matsuo H, Yamada T, Matsumoto K, Sasaki D, Nomura M
- **Journal**: Exp Anim
- **Year**: 2022 (2022-08-05)
- **PMID**: [35264492](https://pubmed.ncbi.nlm.nih.gov/35264492/)
- **DOI**: [10.1538/expanim.21-0187](https://doi.org/10.1538/expanim.21-0187)
- **PMC**: [PMC9388348](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9388348/)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 35264492. https://h2-papers.org/en/papers/35264492
> **Source**: PubMed PMID [35264492](https://pubmed.ncbi.nlm.nih.gov/35264492/)