# Molecular Hydrogen Promotes Adipose-derived Stem Cell Myogenic DifferentiationRegulation of Mitochondria.
> 分子状水素が脂肪由来幹細胞の筋分化を促進するミトコンドリア調節機序の解明


## Abstract

Adipose-derived stem cells (ADSCs) represent an accessible cell source for skeletal muscle regeneration, yet their survival and differentiation capacity are compromised in the oxidative stress-rich microenvironment following transplantation. This in vitro study investigated how molecular hydrogen (H2) influences ADSC biology. Using MTT assays, live-dead staining, western blotting, immunofluorescence, confocal microscopy, and transmission electron microscopy, the researchers found that an appropriate H2 concentration reduced mitochondrial reactive oxygen species (ROS) levels, increased mitochondrial number, and stimulated mitophagy. These mitochondrial changes were associated with improved ADSC survival and enhanced myogenic differentiation efficiency. The findings suggest that H2 may have utility in addressing pathologies linked to mitochondrial dysfunction, including skeletal muscle disorders.

### Mechanism

H2 reduces mitochondrial ROS generation in ADSCs, while simultaneously increasing mitochondrial number and activating mitophagy, collectively enhancing cell survival and myogenic differentiation capacity.

## Bibliographic

- **Authors**: Yang Y, Fei WY, Liu M, Zhang YJ, Gao RS, Hu Y, et al.
- **Journal**: Curr Stem Cell Res Ther
- **Year**: 2023
- **PMID**: [36165523](https://pubmed.ncbi.nlm.nih.gov/36165523/)
- **DOI**: [10.2174/1574888X17666220926115240](https://doi.org/10.2174/1574888X17666220926115240)
- **Study type**: in vitro study
- **Delivery route**: in vitro
- **Effect reported**: positive

## Delivery context

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

## Safety notes

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

---

> **Cite as**: H2 Papers — PMID 36165523. https://h2-papers.org/en/papers/36165523
> **Source**: PubMed PMID [36165523](https://pubmed.ncbi.nlm.nih.gov/36165523/)