# Effect of molecular hydrogen treatment on Sepsis-Associated encephalopathy in mice based on gut microbiota.
> 腸内細菌叢を介した分子状水素による敗血症関連脳症への影響：マウスモデルを用いた検討


## Abstract

Sepsis-associated encephalopathy (SAE) affects up to 70% of critically ill patients with sepsis, yet its pathogenesis remains poorly understood. Male wild-type mice were allocated to four groups: sham, SAE, SAE with 2% H2 gas inhalation, and SAE with hydrogen-rich water. Fecal samples collected 24 hours post-model establishment underwent 16S rDNA sequencing, while serum and brain tissue were subjected to non-targeted metabolomics. H2 administration—via either inhalation or hydrogen-rich water—significantly improved functional outcomes in SAE mice, suppressed inflammatory responses in both brain and gut, corrected gut microbiota dysbiosis, and partially restored metabolic homeostasis. These findings suggest that the beneficial effects of molecular hydrogen on SAE may be mediated through enrichment of beneficial bacterial populations, suppression of pathogenic bacteria, and amelioration of metabolic disturbances.

### Mechanism

Molecular hydrogen appears to restore gut microbiota balance by promoting beneficial bacteria and suppressing harmful species, thereby correcting metabolic dysregulation and reducing neuroinflammation and intestinal inflammation in SAE.

## Bibliographic

- **Authors**: Han Q, Bai Y, Zhou C, Dong B, Li Y, Luo N, et al.
- **Journal**: CNS Neurosci Ther
- **Year**: 2023
- **PMID**: [36468415](https://pubmed.ncbi.nlm.nih.gov/36468415/)
- **DOI**: [10.1111/cns.14043](https://doi.org/10.1111/cns.14043)
- **PMC**: [PMC9873526](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873526/)
- **Study type**: animal study
- **Delivery route**: mixed routes
- **Effect reported**: positive
- **H2 concentration**: 2%

## Delivery context

This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

This study combines multiple delivery routes. As a general principle, the most efficient route for routine hydrogen intake is inhalation. Inhalation carries explosion risk (empirical LFL of 10%; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 36468415. https://h2-papers.org/en/papers/36468415
> **Source**: PubMed PMID [36468415](https://pubmed.ncbi.nlm.nih.gov/36468415/)