# Protective Effect of Hydrogen-Rich Saline on Spinal Cord Damage in Rats.
> 水素富化生理食塩水がラット脊髄損傷に与える保護効果


## Abstract

This animal study examined the effects of intraperitoneally administered hydrogen-rich saline (HRS) on spinal cord injury (SCI) in 4-month-old male Sprague Dawley rats (n=24) divided into four groups: laminectomy-only control, SCI without treatment, HRS alone, and SCI followed by 7-day HRS administration. Spinal cord injury was induced at the T7-T10 vertebral level using the Tator and Rivlin clip compression model for 1 minute with the dura left intact. Blood samples collected on day 7 showed significantly reduced interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) concentrations in the HRS-treated SCI group compared to the untreated SCI group. Histological assessment using hematoxylin-eosin and TUNEL staining further revealed a reduction in apoptotic cell death in spinal cord tissue. These findings suggest that HRS exerts both anti-inflammatory and anti-apoptotic effects in the context of acute spinal cord compression injury.

### Mechanism

Intraperitoneal HRS administration suppressed pro-inflammatory cytokines IL-6 and TNF-α in blood following spinal cord compression, and reduced apoptotic cell death in spinal tissue, indicating anti-inflammatory and anti-apoptotic mechanisms.

## Bibliographic

- **Authors**: Kayabaş M, Şahin L, Makav M, Alwazeer D, Aras L, Yiğit S, et al.
- **Journal**: Pharmaceuticals (Basel)
- **Year**: 2023 (2023-04-01)
- **PMID**: [37111284](https://pubmed.ncbi.nlm.nih.gov/37111284/)
- **DOI**: [10.3390/ph16040527](https://doi.org/10.3390/ph16040527)
- **PMC**: [PMC10143771](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143771/)
- **Study type**: animal study
- **Delivery route**: injection / infusion
- **Effect reported**: positive

## Delivery context

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

## Safety notes

Intravenous hydrogen-saline infusion is a clinic-only route and is not viable for everyday self-administration. For routine hydrogen intake, inhalation is the most practical route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration 66% / 100% devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 37111284. https://h2-papers.org/en/papers/37111284
> **Source**: PubMed PMID [37111284](https://pubmed.ncbi.nlm.nih.gov/37111284/)