# [Hydrogen-rich water reduces cell damage by reducing excessive autophagy in mouse neuronal cells after oxygen glucose deprivation/reoxygenation].
> 酸素グルコース剥奪/再酸素化後のマウス神経細胞における水素水の過剰オートファジー抑制を介した細胞保護効果


## Abstract

This in vitro study examined the cytoprotective properties of hydrogen-rich water (HW) in HT22 mouse hippocampal neuronal cells subjected to oxygen-glucose deprivation/reoxygenation (OGD/R). OGD/R was induced using sodium sulfite in glucose-free medium for 90 minutes followed by 4-hour reoxygenation. Cell viability assessed by CCK-8 dropped to approximately 49% in the OGD/R group compared with 100% in controls; HW exposure restored viability to approximately 63%. Transmission electron microscopy revealed nuclear membrane disruption and elevated autophagic lysosome counts in OGD/R cells, both of which were reduced by HW. Immunofluorescence and Western blotting demonstrated that OGD/R markedly elevated LC3II/I (1.44 vs. 0.37) and Beclin-1 (1.00 vs. 0.64) protein levels relative to controls, whereas HW significantly lowered these autophagy markers (LC3II/I: 0.54; Beclin-1: 0.83). These findings indicate that HW attenuates OGD/R-induced neuronal cell damage, with the mechanism likely involving downregulation of excessive autophagic activity.

### Mechanism

Hydrogen-rich water downregulates OGD/R-induced overactivation of autophagy, evidenced by reduced LC3II/I and Beclin-1 protein expression, thereby limiting autophagic lysosome accumulation and neuronal cell death in HT22 cells.

## Bibliographic

- **Authors**: Li Y, Liu Y, Tao J, Li SC
- **Journal**: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
- **Year**: 2023
- **PMID**: [37366131](https://pubmed.ncbi.nlm.nih.gov/37366131/)
- **DOI**: [10.3760/cma.j.cn121430-20221214-01092](https://doi.org/10.3760/cma.j.cn121430-20221214-01092)
- **Study type**: in vitro study
- **Delivery route**: in vitro
- **Effect reported**: positive

## Delivery context

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

## Safety notes

This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 37366131. https://h2-papers.org/en/papers/37366131
> **Source**: PubMed PMID [37366131](https://pubmed.ncbi.nlm.nih.gov/37366131/)