# Hydrogen-rich water treatment targets RT1-Db1 and RT1-Bb to alleviate premature ovarian failure in rats.
> 水素水投与がRT1-Db1およびRT1-Bbを標的として早発卵巣不全ラットの卵巣障害を軽減する


## Abstract

Using a cyclophosphamide-induced premature ovarian failure (POF) rat model, this study examined the ovarian-protective effects of hydrogen-rich water (HRW). Serum anti-Müllerian hormone (AMH) and 17-β-estradiol (E2) levels rose significantly in HRW-administered animals, while follicle-stimulating hormone (FSH) declined, alongside improved ovarian histomorphology and reduced apoptosis by TUNEL assay. Tandem mass tag (TMT)-based quantitative proteomics identified 16 candidate differentially expressed proteins through cross-comparison of POF vs. control and POF+HRW vs. POF groups. These proteins were enriched across 296 Gene Ontology terms and 36 KEGG pathways. Integration of protein-protein interaction and GeneMANIA network analyses pinpointed RT1-Db1 and RT1-Bb as the principal molecular targets underlying HRW-associated ovarian protection in POF rats.

### Mechanism

HRW modulated the expression of MHC class II-related proteins RT1-Db1 and RT1-Bb in ovarian tissue, associated with reduced apoptosis, improved follicular histomorphology, and restoration of reproductive hormone balance (AMH, E2, FSH) in cyclophosphamide-induced POF rats.

## Bibliographic

- **Authors**: Meng XE, Zhang S, Zhao L, Wang Y
- **Journal**: PeerJ
- **Year**: 2023
- **PMID**: [37397014](https://pubmed.ncbi.nlm.nih.gov/37397014/)
- **DOI**: [10.7717/peerj.15564](https://doi.org/10.7717/peerj.15564)
- **PMC**: [PMC10314742](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10314742/)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

---

> **Cite as**: H2 Papers — PMID 37397014. https://h2-papers.org/en/papers/37397014
> **Source**: PubMed PMID [37397014](https://pubmed.ncbi.nlm.nih.gov/37397014/)