# Hydrogen-rich water improves sleep consolidation and enhances forebrain neuronal activation in mice.
> 水素水がマウスの睡眠統合と前脳ニューロン活性化に与える影響


## Abstract

Sleep deprivation is associated with neurological impairment and broader health consequences. This animal study examined whether hydrogen-rich water (HRW) at concentrations of 0.7–1.4 mM, administered ad libitum for 7 days, could influence sleep architecture and neuronal activity in adult C57BL/6J mice implanted with EEG and EMG electrodes. Under undisturbed baseline conditions, HRW-treated mice showed improved sleep consolidation. Following acute sleep deprivation, HRW-treated animals exhibited increased amounts of both non-rapid-eye-movement and rapid-eye-movement sleep, along with a reduction in the latency to sleep onset after light onset. cFos immunostaining revealed significantly altered neuronal activation in the lateral septum, medial septum, ventrolateral preoptic area, and median preoptic area in HRW-treated mice. These findings suggest that HRW modulates sleep-regulatory neural circuits and may support recovery of sleep quality following sleep loss.

### Mechanism

HRW appears to modulate sleep-regulatory neural circuits by altering cFos-marked neuronal activation in the lateral septum, medial septum, ventrolateral preoptic area, and median preoptic area, thereby improving sleep consolidation and increasing NREM and REM sleep duration in sleep-deprived mice.

## Bibliographic

- **Authors**: Vincent SM, Madani M, Dikeman D, Golden K, Crocker N, Jackson C, et al.
- **Journal**: Sleep Adv
- **Year**: 2024
- **PMID**: [38264142](https://pubmed.ncbi.nlm.nih.gov/38264142/)
- **DOI**: [10.1093/sleepadvances/zpad057](https://doi.org/10.1093/sleepadvances/zpad057)
- **PMC**: [PMC10803172](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10803172/)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 38264142. https://h2-papers.org/en/papers/38264142
> **Source**: PubMed PMID [38264142](https://pubmed.ncbi.nlm.nih.gov/38264142/)