# An ethically guided preclinical device for phenotyping H2 production in laboratory rodents.
> 実験用げっ歯類における内因性水素産生フェノタイピングのための倫理的前臨床デバイスの開発


## Abstract

Intestinal microbiota ferment dietary carbohydrates to generate endogenous dihydrogen (H2), whose production characteristics are of growing preclinical relevance. A welfare-compliant monitoring device was constructed, consisting of a wire cage housed within an airtight chamber equipped with continuous air-quality control and H2 concentration sensing. H2 production rates (HPR) were derived via a first-order moving-average algorithm. Hyperphagia-prone Zucker rats exhibited HPR approximately twice that of Wistar controls (2.64 vs. 1.27 nmol/s per animal). Dietary supplementation with inulin, a fermentable fiber, elevated HPR in mice roughly fourfold compared with control diet (1.99 vs. 0.46 nmol/s per animal). Experiments spanning 12 minutes to 1 hour were conducted repeatedly without compromising animal welfare. The system enables metabolic phenotyping of laboratory rodents based on their endogenous H2 output, providing a quantitative tool for preclinical investigations into gut-microbiota-derived hydrogen.

### Mechanism

Gut microbiota ferment dietary carbohydrates, including fibers such as inulin, generating H2 at rates that vary with the host's metabolic phenotype and dietary composition, as captured by continuous chamber-based sensing.

## Bibliographic

- **Authors**: Pascal-Moussellard V, Boucher E, Tanguy S, Cinquin P, Barraud PA, Davin C, et al.
- **Journal**: Animal Model Exp Med
- **Year**: 2024
- **PMID**: [38925626](https://pubmed.ncbi.nlm.nih.gov/38925626/)
- **DOI**: [10.1002/ame2.12460](https://doi.org/10.1002/ame2.12460)
- **PMC**: [PMC11369032](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11369032/)
- **Study type**: animal study
- **Delivery route**: not specified
- **Effect reported**: not assessed

## Delivery context

The delivery route is not clearly identifiable from this paper. For hydrogen intake, inhalation is the most efficient route; inhalation, however, carries explosion risk (empirical LFL of 10%; high-concentration devices are not recommended).

## Safety notes

The delivery route is not clearly identifiable from this paper. For hydrogen intake, inhalation is the most efficient route; inhalation, however, carries explosion risk (empirical LFL of 10%; high-concentration devices are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)

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> **Cite as**: H2 Papers — PMID 38925626. https://h2-papers.org/en/papers/38925626
> **Source**: PubMed PMID [38925626](https://pubmed.ncbi.nlm.nih.gov/38925626/)