# Hydrogen-Rich Alkaline Water Supplementation Restores a Healthy State and Redox Balance in HO-Treated Mice.
> 水素リッチアルカリ水の摂取が過酸化水素処置マウスにおける酸化還元バランスと健康状態を回復させる


## Abstract

This study examined the effects of hydrogen-rich alkaline water (HAW) on systemic oxidative stress induced by hydrogen peroxide (H₂O₂) administration in mice. Animals received H₂O₂ for two weeks and were either left without supplementation or provided with HAW. HAW-supplemented mice showed decreased plasma reactive oxygen species (ROS) levels alongside elevated circulating glutathione concentrations. A concurrent reduction in plasma 8-hydroxy-2'-deoxyguanosine (8-OHdG) indicated diminished whole-body DNA damage. Analysis of spleen and bone marrow cells revealed lower intracellular ROS content, a significant decrease in mitochondrial membrane potential, reduced superoxide accumulation, and enhanced spontaneous cell proliferation. These findings collectively indicate that HAW can counteract systemic redox imbalance and restore physiological homeostasis under conditions of oxidative toxicity.

### Mechanism

HAW intake reduced plasma ROS and elevated circulating glutathione, while decreasing 8-OHdG levels and DNA damage. In immune cells, mitochondrial membrane potential and superoxide accumulation were suppressed, collectively restoring redox balance.

## Bibliographic

- **Authors**: Mizzoni D, Logozzi M, Di Raimo R, Spada M, Fais S
- **Journal**: Int J Mol Sci
- **Year**: 2024 (2024-06-19)
- **PMID**: [38928440](https://pubmed.ncbi.nlm.nih.gov/38928440/)
- **DOI**: [10.3390/ijms25126736](https://doi.org/10.3390/ijms25126736)
- **PMC**: [PMC11203767](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11203767/)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 38928440. https://h2-papers.org/en/papers/38928440
> **Source**: PubMed PMID [38928440](https://pubmed.ncbi.nlm.nih.gov/38928440/)