# Molecular hydrogen supplementation in mice ameliorates lipopolysaccharide-induced loss of interest.
> 水素補給によるリポ多糖誘発性の興味喪失の改善：マウスモデルを用いた検討


## Abstract

This study examined the effects of hydrogen supplementation on inflammation-related psychiatric symptoms using a mouse model. Institute of Cancer Research mice received 30% hydrogen via jelly intake (40 g/day) for 7 days, followed by intraperitoneal lipopolysaccharide (LPS) administration at 5 mg/kg to induce depressive-like behavior. Behavioral assessments 24 hours post-LPS showed that spontaneous activity decline was attenuated in the hydrogen group. Social interaction testing revealed that reduced engagement with unfamiliar mice observed in LPS-only animals was absent in hydrogen-treated animals. The forced-swim test showed no significant between-group differences. Biochemically, LPS-induced reduction of zonula occludens-1, a tight junction protein at the cerebrovascular barrier, was prevented in the hydrogen group. These findings suggest hydrogen may selectively prevent loss of interest rather than broadly suppressing depressive-like behavior in this inflammation model.

### Mechanism

Hydrogen supplementation may prevent LPS-induced loss of interest by preserving zonula occludens-1 expression at the cerebrovascular barrier tight junctions, thereby maintaining barrier integrity against inflammation-driven disruption.

## Bibliographic

- **Authors**: Koga M, Sato M, Nakagawa R, Tokuno S, Asai F, Maezawa Y, et al.
- **Journal**: PCN Rep
- **Year**: 2024
- **PMID**: [39171191](https://pubmed.ncbi.nlm.nih.gov/39171191/)
- **DOI**: [10.1002/pcn5.70000](https://doi.org/10.1002/pcn5.70000)
- **PMC**: [PMC11337204](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11337204/)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: mixed
- **H2 concentration**: 30%

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 39171191. https://h2-papers.org/en/papers/39171191
> **Source**: PubMed PMID [39171191](https://pubmed.ncbi.nlm.nih.gov/39171191/)