# Molecular hydrogen inhibits neuroinflammation and ameliorates depressive-like behaviors and short-term cognitive impairment in senescence-accelerated mouse prone 8 mice.
> 水素分子が老化促進モデルマウス（SAMP8）における神経炎症・うつ様行動・短期認知障害に及ぼす影響


## Abstract

SAMP8 senescence-accelerated mice received hydrogen-rich jelly (HRJ) or placebo jelly from 6 weeks of age for 26–28 weeks. Behavioral assessments showed that HRJ reduced immobility in tail suspension and forced swimming tests, and improved spatial working memory and visual recognition in Y-maze and object recognition tasks. Immunohistochemical analysis of the medial prefrontal cortex and hippocampal dentate gyrus revealed decreased expression of 8-OHdG, Iba1, and cleaved caspase-3 in HRJ-treated animals. ELISA measurements further demonstrated significantly lower IL-6 concentrations in brain tissue of SAMP8 mice given HRJ compared with placebo controls. Collectively, these findings indicate that molecular hydrogen suppresses microglial activation and associated neuroinflammation, leading to attenuation of depressive-like behavior and short-term cognitive decline in an accelerated aging mouse model.

### Mechanism

Molecular hydrogen suppresses microglial activation, reducing oxidative DNA damage marker 8-OHdG, Iba1 expression, cleaved caspase-3, and IL-6 production in brain tissue, thereby attenuating neuroinflammation in aging mice.

## Bibliographic

- **Authors**: Nakagawa K, Kodama K, Nagata W, Takahashi S, Satoh Y, Ishizuka T
- **Journal**: Behav Brain Res
- **Year**: 2025 (2025-02-26)
- **PMID**: [39522774](https://pubmed.ncbi.nlm.nih.gov/39522774/)
- **DOI**: [10.1016/j.bbr.2024.115330](https://doi.org/10.1016/j.bbr.2024.115330)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 39522774. https://h2-papers.org/en/papers/39522774
> **Source**: PubMed PMID [39522774](https://pubmed.ncbi.nlm.nih.gov/39522774/)