# Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer's disease: targeting oxidative stress, inflammation, and the gut-brain axis.
> ゼブラフィッシュのアルツハイマー病モデルにおける水素水の効果：酸化ストレス・炎症・腸脳軸への影響


## Abstract

Using a zebrafish model of Alzheimer's disease (AD) induced by aluminum chloride exposure, this study examined the effects of hydrogen-rich water (HRW) delivered via a nanobubble device. Behavioral assessments, ELISA, hematoxylin-eosin staining, ROS and neutrophil fluorescence labeling, and 16S rRNA sequencing were applied. HRW significantly ameliorated cognitive impairment and depression-like behavior, reduced amyloid-beta (Aβ) deposition (p<0.0001), and modulated hepatic soluble epoxide hydrolase (sEH) levels (p<0.05). Neuroinflammation and oxidative stress were also attenuated. Additionally, HRW restored gut microbiota balance, lowering the abundance of bacteria associated with AD pathology. These results indicate that HRW exerts multi-target effects encompassing antioxidant, anti-inflammatory, and gut-brain axis regulatory actions in this AD model.

### Mechanism

HRW suppressed Aβ-induced ROS generation and oxidative stress, modulated hepatic soluble epoxide hydrolase (sEH) to reduce neuroinflammation, and restored gut microbiota composition, thereby influencing the gut-brain axis in an AD zebrafish model.

## Bibliographic

- **Authors**: He J, Xu P, Xu T, Yu HS, Wang L, Chen R, et al.
- **Journal**: Front Aging Neurosci
- **Year**: 2024
- **PMID**: [39839307](https://pubmed.ncbi.nlm.nih.gov/39839307/)
- **DOI**: [10.3389/fnagi.2024.1515092](https://doi.org/10.3389/fnagi.2024.1515092)
- **PMC**: [PMC11746902](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11746902/)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 39839307. https://h2-papers.org/en/papers/39839307
> **Source**: PubMed PMID [39839307](https://pubmed.ncbi.nlm.nih.gov/39839307/)