# Silicon-based agent mitigates fatty liver formation in a CDAHFD60-induced MASH mouse model by enhancing hepatic function.
> シリコン系製剤によるMASHマウスモデルにおける脂肪肝形成の抑制と肝機能改善効果


## Abstract

Metabolic dysfunction-associated steatohepatitis (MASH) is a progressive hepatic condition driven by oxidative stress and fat accumulation, with rising global incidence linked to obesity and metabolic syndrome. A silicon (Si)-based agent that continuously produces molecular hydrogen upon contact with water was evaluated in a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD60)-induced MASH mouse model. The agent was incorporated at 2.5% into the diet and administered for 12 weeks. Despite the absence of macroscopic changes in liver appearance, histological analysis revealed reduced hepatic lipid droplet accumulation, attributed to amelioration of MASH-associated bile dysfunction. The Si-based agent also alleviated the hypolipidemia and body weight loss characteristic of this model, and attenuated systemic oxidative stress markers. Collectively, these findings indicate that continuous endogenous hydrogen generation via the Si-based agent improves both hepatic and systemic lipid metabolism while exerting antioxidant effects in MASH.

### Mechanism

The Si-based agent reacts with water to continuously generate molecular hydrogen, which scavenges reactive oxygen species, thereby improving bile function, reducing hepatic lipid droplet accumulation, and attenuating systemic oxidative stress in MASH.

## Bibliographic

- **Authors**: Koyama Y, Kobayashi Y, Hirota I, Kobayashi H, Shimada S
- **Journal**: Biochem Biophys Rep
- **Year**: 2026
- **PMID**: [41890216](https://pubmed.ncbi.nlm.nih.gov/41890216/)
- **DOI**: [10.1016/j.bbrep.2026.102552](https://doi.org/10.1016/j.bbrep.2026.102552)
- **PMC**: [PMC13014671](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13014671/)
- **Study type**: animal study
- **Delivery route**: hydrogen-rich water
- **Effect reported**: positive
- **H2 concentration**: 2.5%

## Delivery context

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

## Safety notes

Hydrogen-rich water is a low-risk delivery route, but the achievable systemic hydrogen dose is bounded. For clinical applications, inhalation is the most efficient route; inhalation, however, carries explosion risk, and concentration matters (empirical LFL of 10% applies to inhalation environments; high-concentration devices are documented in the Consumer Affairs Agency accident database and are not recommended).

See also:
- [Inhalation concentration and LFL / UFL](https://h2-papers.org/en/safety-notes/inhalation-concentration)
- [Consumer Affairs Agency accident cases](https://h2-papers.org/en/safety-notes/accident-cases)
- [Inhalation safety threshold lineage](https://h2-papers.org/en/safety-notes/lineage)

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> **Cite as**: H2 Papers — PMID 41890216. https://h2-papers.org/en/papers/41890216
> **Source**: PubMed PMID [41890216](https://pubmed.ncbi.nlm.nih.gov/41890216/)