窒素反応ガスを用いたCRIMSによる水素・重水素測定の新手法
A novel analytical approach using chemical reaction interface mass spectrometry (CRIMS) with nitrogen as the reactant gas is described for quantifying deuterium isotopic enrichment. The reaction generates molecular hydrogen species (H2 and HD), enabling deuterium content analysis with a benchtop quadrupole mass spectrometer. Deuterium-labeled leucine served as the primary test compound, and measurements demonstrated high accuracy, precision, and linearity. The method was further applied to assess acetylation of lysine residues in the peptide neurotensin; C18 column separation revealed a 61% yield of the monoethylated product with a D/H ratio closely matching the theoretical value. The authors propose that deuterium-based isotope ratio monitoring via CRIMS offers a cost-effective and synthetically simpler alternative to 13C or 15N labeling for metabolic tracer studies, provided that deuterium kinetic isotope effects are not a concern.
Nitrogen reactant gas in the chemical reaction interface converts organic compounds into molecular hydrogen species (H2 and HD), allowing deuterium-to-hydrogen ratio determination by quadrupole mass spectrometry.
This is basic research at the cellular or molecular level. For human application, inhalation is the most promising delivery route, but inhalation carries explosion risk and concentration matters (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/10790843