ポリヒドロキシアルカノエートを緩効性炭素源とした汚染帯水層の原位置バイオレメディエーション：室内試験からパイロットスケール現場試験まで
This study examined the feasibility of using poly-3-hydroxy-butyrate (PHB) as an electron donor for in situ biological reductive dechlorination of chlorinated aliphatic hydrocarbons (CAHs) in groundwater. PHB undergoes a restricted fermentation pathway producing acetic acid and molecular hydrogen, thereby minimizing residual carbon accumulation that could degrade groundwater quality. Prior to field deployment at a heavily CAH-contaminated industrial site in Italy, microcosm experiments confirmed quantitative conversion of CAHs to ethene. A 30-m-deep, 3-screened groundwater circulation well (GCW) coupled with an external treatment unit was installed to distribute PHB fermentation products into low-permeability zones. Quantitative real-time PCR monitoring over the first 4 months of operation revealed up to a 6.6-fold increase in Dehalococcoides mccartyi abundance and measurable reductions in CAH concentrations at monitoring wells, confirming effective delivery and stimulation of dechlorination activity.
PHB fermentation yields acetic acid and molecular hydrogen, which serve as electron donors supporting Dehalococcoides mccartyi-mediated reductive dechlorination of chlorinated hydrocarbons in the subsurface.
The delivery route is not clearly identifiable from this paper. For hydrogen intake, inhalation is the most efficient route; inhalation, however, carries explosion risk (empirical LFL of 10%; high-concentration devices are not recommended).
See also:
https://h2-papers.org/en/papers/27903429